rapC [2018-11-14 09:23:23]

response regulator aspartate phosphatase, controls [protein|search|ComA ]activity

locus: BSU03770

pI: 5.02

mw: 45.47 kDa

protein length: 382 aa

gene length: 1146 bp

function: control of [protein|search|ComA ]activity

product: response regulator aspartate phosphatase

essential: no

synonyms: yclL

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.2|Response regulator aspartate phosphatase]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]

Gene

Coordinates: 428,831 → 429,979

The protein

Catalyzed reaction/ biological activity

• control of [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA] activity [Pubmed|12950917]

Protein family

• RAP family (according to Swiss-Prot)

[SW|Domains]

• five [SW|TPR repeat|tetratrichopeptide repeats] (according to UniProt)

Effectors of protein activity

• binding of [protein|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|PhrC] to [protein|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|RapC] prevents it from binding [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA] [Pubmed|12950917]

Structure

• [PDB|4I9E] ([protein|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|RapF], 57% identity) [pubmed|23526880]

Expression and Regulation

Operons

• genes: [gene|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|rapC]-[gene|C0B7845F55A16300EB37F026C817AEC4F3BA3B6F|phrC]
  description: [Pubmed|10464187]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10464187], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|10464187], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|10464187], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]) [Pubmed|16091051], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expressed at high cell density ([protein|search|ComA]) [Pubmed|16091051]

Biological materials

Mutant

• MGNA-C065 (rapC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2063 NBRP B. subtilis, Japan]
• BKE03770 (Δ[gene|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|rapC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTTCACCCTCTCCCA, downstream forward: _UP4_CAAATACAAAGGAGTGAAGG
• BKK03770 (Δ[gene|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|rapC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTTCACCCTCTCCCA, downstream forward: _UP4_CAAATACAAAGGAGTGAAGG

References

• 12950917,16091051,10464187,26582911,23526880